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【Detection of new corona virus（COVID-19）】

Dohgen Corporation is developing a novel system of detection of new corona virus in collaboration with TSUKUBA GENE-TECHNOLOGY Laboratories, Inc.(Details）

★★Primer kits for the detection of new corona virus (COVID-19) to be planned★★

【Detection method of new corona virus (COVID-19)】
　　★　Survey of conserved sequence among the Covid-19 sequences registered in the Genbank
　　★　Design the primer sets to produce the PCR product up to 150bp
　　★　The primer (designated as reverse primer) for reverse transcription from viral RNA to cDNA
　　　　　is to be added with a Tag sequence.
　　★　In the first process of the Covid-19 detection, RNA is extracted from biological samples
　　　　　and used as a temperate for reverse transcription
　　★　In the reverse transcription, viral RNA is transformed to cDNA using the reverse primer and reverse transcriptase.
　　★　The cDNA thus obtained is subjected to real time PCR using the forward primer and the tag-primer
　　　　　to measure the amount of cDNA in the samples. The amount of cDNA corresponds directly to that
　　　　　of viral RNA in the samples.
　　　　　　
【Schematic presentaion of detection of new corona virus (COVID-19)】
　　
　　　

　【Primer sequences for detection of new corona virus】
　　　　

　　　【Conclusion】 Our system can detect COVID-19
　　　　　　　　　　　if there is only 2 molecules of COVID-19 viral RNA in a sample.
　　

　【Components of primer kit for detection of COVID-19】　
　　　1）　Forward Primer
　　　2）　Reverse Primer
　　　3）　Positive control
　
　　　Please contact us!（Contact ）。

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